Protein Crosslinking

Art. No.

E030

Background info

The ZediXplore EasyGel Kit contains the materials required for preparing stable, crosslinked, gelatin-based gels.
Both components, X Pure Gelatin® and Microbial Transglutaminase Andracon®, have been tested for endotoxins.
The kit enables the generation of X Pure Gelatin® gels, which are stabilized by Andracon® through the formation of covalent crosslinks. 
The conditions given below are recommended as a starting point for preparing crosslinked gelatin gels. Adaptation of these conditions to fulfil the requirements of specific applications may be necessary.
The reaction buffer is not included in the kit. Typically, the buffer which is part of the subsequent application can be used, which is often phosphate buffered saline, pH 7.2.

Reagents in the kit

4 x 250 mg X Pure Gelatin® (ultra-purified clinical grade gelatin, X)
1 x 25 U Andracon® (Microbial Transglutaminase, MTG, A)

Application

Kit for Preparation of Crosslinked Gelatin Hydrogels using X Pure Gelatin® and Andracon®

Storage

Store Andracon® (A) at ≤ -20°C (shipment at ambient temperature is possible). 
Store X Pure Gelatin® (X) at room temperature.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.