Andracon™ – Recombinant Microbial Transglutaminase (HEPES-formulated, lyophilized)
EC 22.214.171.124; Protein-glutamine-γ-glutamyltransferase
AndraconTM is a brand for high quality microbial transglutaminase:
• Recombinantly produced in E. coli
• Ultra-pure and highly active
• Batch to batch consistent quality
• License free
• Produced according to an SOP in an ISO9001:2015 certified environment
AndraconTM (Microbial Transglutaminase, MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide-bound glutamine residues and a variety or primary amines.
Therefore, AndraconTM can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.
Essentially, AndraconTM is used for the production of antibody drug conjugates (ADCs).
38334.0 Da (theoretical, activated MTG with N-terminal sequence: FRAPDSDDR...)
Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.
> 30 U/mg
White lyophilized solid.
The purified transglutaminase is lyophilized from 50 mM HEPES pH 7.4.
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
Labeling, immobilisation, conjugation, and modification of proteins.
Store at -80°C. Upon reconstitution, store working aliquots undiluted.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended.
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INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.