Bacterial Pro-transglutaminase (Pro-BTG, recombinantly produced in E. coli,
gene derived from Streptomyces mobaraensis)
EC 188.8.131.52; Protein-glutamine-γ-glutamyltransferase
Recombinantly produced in E. coli
> 90% [SDS-PAGE and Coomassie staining]
10 μl His6-rProTG (1 μg/μl)
35 μl 50 mM Tris-HCl pH 8
5 μl Dispase II (Roche) (0.2 μg/μl)
Incubate for 20 min at 30°C
13 U/mg [One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
White lyophilized solid
The Protransglutaminase is lyophilised from 10 mM Tris-HCl pH 8.0, 150 mM NaCl.
Add the desired volume H2O to the vial of lyophilized powder.
Labelling, immobilisation, conjugation and modification of proteins.
Store at –20°C in working aliquots. Repeated freez ing and thawing is not recommended.
Delivery at ambient temperature is possible
Pasternack et al., Eur. J. Biochem. 1998, 257:570-6
Intended for research use only, not for use in human, therapeutic or diagnostic applications.