Microbial (Pro)TGase

Bacterial Pro-transglutaminase

(Pro-BTG, recombinantly produced in E. coli,
gene derived from Streptomyces mobaraensis)
Quantity Unit Price Status
250 µg 300 € Available
Documents
Art. No.
T016
Synonym
EC 2.3.2.13; Protein-glutamine-γ-glutamyltransferase
Molecular Weight
43 kDa
Source
Recombinantly produced in E. coli
Purity
> 90% [SDS-PAGE and Coomassie staining]
Activation
10 μl His6-rProTG (1 μg/μl)
35 μl 50 mM Tris-HCl pH 8
5 μl Dispase II (Roche) (0.2 μg/μl)
Incubate for 20 min at 30°C
Specific Activity
13 U/mg [One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
Appearance
White lyophilized solid
Reagents
The Protransglutaminase is lyophilised from 10 mM Tris-HCl pH 8.0, 150 mM NaCl.
Reconstitution
Add the desired volume H2O to the vial of lyophilized powder.
Application
Labelling, immobilisation, conjugation and modification of proteins.
Storage
Store at –20°C in working aliquots. Repeated freez ing and thawing is not recommended.
Delivery at ambient temperature is possible
Reference(s)
Pasternack et al., Eur. J. Biochem. 1998, 257:570-6
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.

News

Blog

Events