Microbial (Pro)TGase

Recombinant microbial (bacterial) transglutaminase

(MTG, recombinantly produced in E. coli, gene derived from Streptomyces mobaraensis)
Quantity Unit Price Status
25 U 270 € Available
250 U (1 vial) 2140 € Available
10 x 25 U 2140 € Available
500 U (1 vial) 3750 € Available
20 x 25 U 3750 € Available
Documents
Art. No.
T001
Synonym
EC 2.3.2.13, MTG, BTG, BTGase, Protein-glutamine-γ-glutamyltransferase
Background info
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These
enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.

Microbial Transglutaminase by Zedira
Molecular Weight
38,333 Da (Dispase-activated MTG with N-terminal sequence: FRAPDSDDR...)
Source
Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.
Purity
> 95 % (SDS-PAGE)
Specific Activity
> 25 U/mg [One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
Appearance
White lyophilized solid.
Reagents
The purified transglutaminase is lyophilized from 50 mM NaOAc pH 5.0 + 0.3 M NaCl.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
Reconstituted MTG solution might be turbid. However, MTG activity is not affected. In this case, centrifuge the solution and collect the supernatant.
After reconstitution, the solution should be stored frozen in working aliquots.
Application
Labeling, immobilisation, conjugation and modification of proteins.
MTG can tolerate up to 10% DMSO in aqueous solutions without significant influence on its catalytic activity. A small activity loss of 4% was observed in the presence of 20% DMSO.
Storage
Store at –20°C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature.
Reference(s)

Spycher et al., ChemBioChem 2017, 18:1923-7
Steffen et al., J. Biol. Chem. 2017, 292:15622-35
Dennler et al., Chembiochem. 2015, 16:861-7
Dennler et al., Bioconjugate Chem. 2014, 25, 569-78
Kaufmann et al., Food Addit. Contam. Part. A 2012, 29:1364-73
Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49:9995-7
Gianfrani et al., Gastroenterology 2007, 133:780-9
Pfleiderer et al., Microbiol. Res. 2005, 160:265-71
Ando et al., Agric. Biol. Chem. 1989, 53:2613-17
Pasternack et al., Eur. J. Biochem. 1998, 257:570-6
Grossowicz et al., J. Biol. Chem. 1950, 187, 111-25

Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Process Change Notification: Transfer of the Pro-MTG production protocol from shaker flask to stirred tank fermenter (T001 (MTG) Process Change Notification.pdf).

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