Proteases

Art. No.

D017

Synonym

(D)-Phe-Pip-Arg-AMC

Molecular Formula

C31H39N7O5

Molecular Weight

589.69

Purity by HPLC

>95 % (214 nm)

Appearance

Pale yellow solid

Solubility

50 μM in 0.1% (v/v) DMSO / aqueous buffers Pre-dissolve e.g. 10 mg (17.0 μmol) in 339 μl DMSO - dilute e.g. 10 μl of that stock solution (50 mM) with 9,990 μl aqueous buffer to obtain a 50 μM solution. DMSO stock solutions can be stored at -20°C for at least 6 months. To avoid too many freeze thaw cycles, we strongly recommend storage of aliquots.

Application

Highly sensitive fluorogenic thrombin substrate Lower limit of detection: 5 pM (picomolar) thrombin

Storage

Store at -20°C, desiccate

Reference(s)

Larsen M.L. et al. Thromb. Res. 1978, 13, 285-288. Goodnight S.H. Jr. et al. Am. J. Clin. Pathol. 1980, 73, 639-647. Rijkers D.T. et al. Thromb. Res. 1995, 79, 491-499. Gram, J. and Jespersen, J. Clinical Chemistry 1995, 31, 1683-1688.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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