We measure activity in your transglutaminase samples!

Background: 
Transglutaminase (Microbial transglutaminase, MTG) is a processing aid widely used to improve meat, fish, bakery, and dairy products. Transglutaminase cross-linking can modulate the physical and textural properties of various protein-containing foods. 

Transglutaminase Activity: 
At Zedira, we determine the activity of your transglutaminase sample on a fee-for-service basis. 


 

How to get your sample measured at Zedira?

 

1. Sample shipment 
   Send us at least 50 g of your sample using a carrier of your choice. 
   Ship it to: Zedira GmbH, Roesslerstrasse 83, 64293 Darmstadt, Germany. 
   Please inform us via e-mail about your shipment (contact@zedira.com) and provide the expected activity range.
   Samples typically are MTG concentrates (2,000 – 3,000 U/g) or ready-to-use MTG formulations (60 – 200 U/g). 
   
    
2. Sample measurement 
   The transglutaminase activity of your samples is measured using the standard hydroxamate assay (see assay principle below). 
   Two independent measurements, in triplicate, are performed per sample. 
   Our internal quality standards require inter-assay and intra-assay deviations of no more than 5%.
   
    
3. Results 
   The transglutaminase activity of each sample is stated in a Certificate of Analysis (example for download). 
   The certificate of analysis is sent to you via e-mail within 1-2 working days after receipt of your sample.
   
    
4. Fee for service 
   The assay fee per sample is 275 €. 
   When sending 3 samples at the same time, the fee per analysis is reduced to 247.5 €. 
   The invoice is sent by e-mail and can be paid by bank transfer or credit card.
    

Please contact us for further questions.

Phone: +49 6151 66628-0
E-mail: contact@zedira.com


The transglutaminase activity assay principle:
The Standard Hydroxamate Assay uses Z-Gln-Gly-OH as the peptidic glutamine substrate and hydroxylamine as the amine donor.
In the presence of MTG, hydroxylamine is enzymatically incorporated into the peptide to form Z-glutamyl-hydroxamate-glycine. The hydroxamate forms a red colored complex with iron (III) ions, which is quantified at 525 nm.
One unit of microbial transglutaminase activity is defined as the amount of enzyme, that causes the formation of 1.0 μmole of hydroxamate per minute at 37°C (Folk and Cole, 1966).


(FAQ - Determination of MTG activity.pdf)