Factor XIII

Art. No.

T070

Synonym

Recombinant Fibrin stabilizing factor, protein-glutamine-γ-glutamyltransferase

Molecular Weight

79 kDa (monomer); 158 kDa (homodimer)

Source

Recombinantly produced in insect cells.
Activated by human Thrombin

Purity

> 95% [by SDS-PAGE]

Appearance

White lyophilized solid.

Description

Recombinant human Factor XIII is a homodimer (A2) composed of two chains held together by non covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (Factor XIIIa), Factor XIIIa catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross linked fibrin is insoluble and resistant to lysis.

Reconstitution

Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.

Application

hFXIIIa catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given. Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Storage of diluted aliquots may result in severe activity loss. Avoid repeated freezing and thawing. Delivery at ambient temperature is possible

Reference(s)

Chrobok et al., PLoS One. 2018, 13:e0196433; Akbar et al., J. Med. Chem. 2017, 60:7910-27; de Jager, M. et al., Neuropathol. Appl. Neurobiol. 2016, 42, 255; Hamedani et al., Chem. Commun. 2015, 51, 1135-39; Böhm et al., J. Med. Chem. 2014, 57, 10355-65

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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