Transglutaminase 2

Art. No.

T022

Synonym

Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase

Molecular Weight

78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)

Source

Recombinant produced in insect cells

Purity

> 90 % (visually by SDS-PAGE)

Activation

The Transglutaminase is activated with 10 mM Ca2+

Activity

> 1500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Appearance

White lyophilized solid.

Reconstitution

Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in undiluted working aliquots.

Application

His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given. Store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Avoid repeated freezing and thawing. Delivery is possible at ambient temperature

Reference(s)

Engstrom et al., Sci. Rep. 2017, 7:77 Hardes et al., Anal. Biochem. 2012, 428:73-80

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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