Transglutaminase 4

Art. No.

T042

Synonym

Prostate Transglutaminase, TG4, TGase 4

Molecular Weight

78 kDa

Source

Recombinantly produced in E. coli

Purity

> 95 % (visually by SDS-PAGE)

Activation

Add 10 mM Ca2+ to activate His6-rhTG4.

Activity

[Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG4 catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)]. 1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured with Transglutaminase Assay Kit, Zedira, Product code T036].

Appearance

White lyophilized solid.

Description

His6-rhTG4 is based on clone IMAGp958A10818Q2. It is N-terminally fused to a hexahistidine tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEELLL…. His6-rhTG4 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.

Reconstitution

Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.

Application

His6-rhTG4 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG4 may also be used for immunoprecipitation.

Storage

Store at ≤ -20 °C in working aliquots. Repeated freezing and thawing is not recommended. Delivery is possible at ambient temperature

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T036: Transglutaminase Assay Kit ("DCC"), fluorescent
T101: 1,3,4,5-Tetramethyl-2-[(2-oxopropyl)thio]imidazolium chloride
A022: Polyclonal antibody to human prostate transglutaminase (TG4)