Transglutaminase 6

Art. No.

T021

Synonym

Neuronal Transglutaminase, TGy, TG6, TGase 6, proteinglutamine-γ-glutamyltransferase

Molecular Weight

78 kDa

Source

Recombinantly produced in insect cells

Purity

> 95% [SDS-PAGE and Coomassie staining] The gel shows a single dominant protein band at ~80 kDa.

Activation

His₆-rhTG6 is a Ca²⁺-dependent enzyme.

Appearance

White lyophilized solid.

Reconstitution

Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.

Application

His6-rhTG6 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given. Store working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Repeated freezing and thawing is not recommended. Delivery at ambient temperature is possible

Reference(s)

Fukui et al., FEBS J. 2013, 280:1420-9; Schaertl et al., J. Biomol. Screen. 2010, 15:478-87

Note

TG6 IS INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

A031: FITC-labeled polyclonal antibody to human TG6
T036: Transglutaminase Assay Kit ("DCC"), fluorescent