Transglutaminase 7

Art. No.

T011

Synonym

TG7, TGase 7

Molecular Weight

81 kDa

Source

Recombinantly produced in E. coli

Purity

> 90 % (visually by SDS-PAGE)

Activation

Add 10 mM Ca2+ to activate His6-rhTG7.

Specific Activity

> 1000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His₆-rhTG7-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Appearance

White lyophilized solid.

Description

His6-rhTG7 is based on the TGM7-gene on plasmid pCRII-hTGz cl.14 (isolated by Daniel Aeschlimann), corrected by the insertion of a C at position 1169. It is N-terminally fused to a hexahistidine-tag. His6-rhTG7 is a Ca2+-dependent enzyme.

Reconstitution

Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.

Application

His6-rhTG7 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(ε-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG7 may also be used for immunoprecipitation.

Storage

Store at ≤ - 20 °C. Store working aliquots at ≤ - 20 °C. Avoid repeated freezing and thawing. Delivery at ambient temperature is possible

Reference(s)

Kuramoto at al., Arch: Biochem Biophys. 2013; 537138-43; Fukui et al., FEBS J. 2013, 280:1420-9

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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