Photometric Activity Assay

Art. No.

Z010

Synonym

ZediXclusive Tissue Transglutaminase Assay Kit

Background info

TG2 is present in various tissues and involved in a plentitude of physiological as well as pathological processes. The enzyme catalyses the acyl transfer reaction between the γ-carboxyamide group of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine (Lorand L. et al., 1962). This assay enables the measurement of TG2 activity according to the chromogenic hydroxamate detection principle (Grossowicz, N. et al., 1950).

Description

Chromogenic Assay for measurement of tissue transglutaminase (tTG) activity. The Tissue Transglutaminase Assay Kit uses Z-QQPF as the amine acceptor substrate and hydroxylamine as amine donor. In the presence of tTG hydroxylamine is incorporated into Z QQPF to form Z-glutamyl-hydroxamate-QPF which develops a colored complex with iron (III) detectable at 525 nm.

Assay principle

The TISSUE TRANSGLUTAMINASE ASSAY KIT uses Z-QQPF as the amine acceptor substrate and hydroxylamine as amine donor. In the presence of tTG hydoxylamine is incorporated into Z-QQPF to form Z-glutamylhydroxamate-QPF which develops a colored complex with iron (III) detectable at 525 nm.

Reagents in the kit

(1A) ACTIVITY REAGENT 1A: lyophilized MOPS buffer pH 7.6 containing Z-QQPF, DTT and calcium, 3 vials
(2A) ACTIVITY REAGENT 2A: Hydroxylamine, 3 vials
(3S) STOP REAGENT 3S: Hydrochloric acid [4% v/v], Iron (III) chloride, 3 vials
(4P) TG2 POSITIVE CONTROL 4P: lyophilized TG2, 3 vials

Equipment

The TISSUE TRANSGLUTAMINASE ASSAY KIT can be used in standard spectrophotometers with polystyrene 1 mL cuvettes or MTP. Refer to the instructions of the manufacturer.

Intended use

Determination of tissue transglutaminase activity (tTG, TG2). Kit is sufficient for 3 x 11 measurements in cuvettes or 3 x 36 measurements in 96 well microtiter plates (MTP).

Storage

Store 1A, 2A and 3S at 4-8 °C. 4P must be stored at ≤ -20°C

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T002: Human tissue transglutaminase
T022: Human tissue transglutaminase