FXIII Activity Assays

FXIII-Assay Substance,

Abz-NE(CAD-DNP)EQVSPLTLLK-OH
Quantity Unit Price Status
10 mg 595 € Available
Documents
Art. No.
A101
Background info
Also suitable for TG1, TG2, TG3 and TG6

Factor XIII FXIII assay substance A101 by Zedira
Molecular Formula
C78H122N20O25 (free base)
Molecular Weight
1739.92 (free base)
Purity by HPLC
>95 % (HPLC)
Appearance
yellow solid
Solubility
50 μM in 0.1% (v/v) DMSO / aqueous buffers.
Pre-dissolve e.g. 10 mg (5.39 μmol) in 108 μl DMSO - dilute e.g. 10 μl of that stock solution (50 mM) with 9990 μl aqueous buffer to obtain a 50 μM solution. DMSO stock solutions are sterile and can be stored at -20°C for at least 6 months. To avoid too many freeze-thaw cycles, we strongly recommend storage of aliquots.
Application
Kinetic measurement of transglutaminases
Storage
Store at -20°C, desiccate
Reference(s)
Oertel, K. et al. Anal. Biochem. 2007, 367, 152. 
Dodt, J. et al. Anal. Biochem. 2013, 439, 145. 
Berny‐Lang, M.A. et al. J. Am. Heart Assoc. 2013, 2, e000026. 
Schroeder, V. et al. Br. J. Haematol. 2015, 168, 757. 
de Jager, M. et al. Neuropathol. Appl. Neurobiol. 2016, 42, 255. 
Király, R. et al. Amino Acids 2016, 48, 31. 
van der Wildt, B. et al. Nucl. Med. Biol. 2016, 43, 232. 
Dodt, J. et al. Br. J. Haematol. 2016, 172, 452. 
Chrobok, N.L. et al. PLoS ONE 2018, 13, e0209522. 
Akbar, A. et al. J. Med. Chem. 2017, 60, 7910-7927. 
Singh, S. et al. Int. J. Mol. Sci. 2019, 20, 2682. 
Gates et al. Molecules 2023, 28, 1634. 
Hamedani, N.S. et al. J. Clin. Med. 2021, 10, 677.
Note
The quantification of the TFA counter ion content is performed by using a 19F NMR method according to Little et al. (J. Pharm. Biomed. Anal. 2007, 43, 1324), based on an internal standard.

Intended for research use only, not for use in human, therapeutic or diagnostic applications.

News

Blog

Events