Recombinant Gliadin Peptides

Art. No.

G007

Synonym

26mer γ-gliadin, deamidated; 26mer DGP (Corresponds to Zedira product No. G053); Deamidated Gliadin Peptide – fusion protein

Background info

Detection of gliadin antibodies has been used for a long time in celiac disease diagnostics but suffered from a low specificity. This disadvantage was overcome by the introduction of deamidated gliadin peptides as antigen. The rationale behind is that tissue transglutaminase catalyzes gliadin deamidation in the intestinal mucosa of celiac disease patients, resulting in deamidated gliadin peptides which are recognized by HLA receptors of immune cells. Therefore, deamidated gliadin antibodies are specific for celiac disease. We introduced four different variations of deamidated gliadin antigens composed by a carrier protein linked with a combination of the deamidated 33-mer and 26-mer gamma gliadin peptides and the DQ2-GI- and DQ2-GII-peptides (Dørum S. et al., J Proteome Res. 2009; 8:1748-55). In addition, the non-deamidated native versions as well as the carrier protein control are also available

Molecular Weight

25.77 kDa

Source

Recombinantly produced in E. coli

Purity

> 95% (SDS-PAGE and Coomassie staining)

Appearance

White lyophilized solid.

Description

DGPx1 is a fusion protein of a deamidated gliadin peptide fused with a carrier protein to be used as antigen for the detection of antibodies specific for deamidated gliadin. The deamidated gliadin peptide is based on the 26-mer gamma gliadin (Dørum S. et al., J Proteome Res. 2009; 8:1748-55).

Reconstitution

Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution, the solution should be stored frozen in working aliquots.

Application

The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is recognized y human type IgA and IgG antibodies.

Coating

Dilute DGPx1 with your coating buffer to an appropriate concentration e.g. 1 µg/ml. Please note that coating conditions have to be evaluated carefully.

Storage

Store lyophilized DGPx1 at -80°C. Stability is given for at least 4 years when stored at -80°C (see retest date on Certificate of Analysis), with potential to date extend after retesting. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Solutions of DGPx1 are stable for at least 2 years when stored at ≤ -20°C. Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Avoid repeated freezing and thawing. Delivery is possible at ambient temperature

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

G005: DGPx4
G006: DGPx2