2-[(2-oxopropyl)thio]
imidazolium derivates

Art. No.

D004

Synonym

“L-683.685”; 1,3-Dimethyl-2-(2-oxopropylthio)-4,5-diphenyl-1H-imidazol-3-ium trifluoromethanesulfonate

Molecular Formula

C21H21F3N2O4S2 (free cation: C20H21N2OS+)

Molecular Weight

486.53 (free cation: 337.46)

Purity by HPLC

>90 % (214 nm)

Purity

> 95% (NMR)

Appearance

Off white solid

Solubility

Pre-dissolve e.g. 2.02 mg in 207.6 µl DMSO to obtain a 20 mM stock solution - dilute 20 µl of that stock solution with 980 µl buffer (e.g. 50 mM TRIS-HCl, pH 7.5) to obtain a 400 µM solution

Application

Inhibitior for transglutaminase, cell-permeable [3] / INHIBITION OF PURIFIED TISSUE-TGase (HIS6-rhTG2) 1,3-Dimethyl-4,5-diphenyl-2-[(2-oxopropyl)thio]imidazolium, trifluorosulfonic acid salt is a potent, active site directed inhibitor. The reaction results in an acetonylation of active site cysteine. The inhibition parameters were obtained by progress curve analysis using a continuous assay after preincuabation with the inhibitor (Fig. 1a and 1b).

Storage

Store at -20°C, desiccate

Reference(s)

[1] Lorand L. et al. Exp. Eye Res. 1998, 66, 531. [2] Barsigian C. et al. J. Biol. Chem. 1991, 266, 22501. [3] Basso, M. et al. J. Neurosci. 2012, 32, 6561.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

A101: FXIII-Assay Substance,
T002: Human tissue transglutaminase
T027: Human blood coagulation Factor XIII-A₂