„DON“ compounds

Art. No.

Z006

Synonym

Z-DON-Val-Pro-Leu-OMe, “Z-DON”

Molecular Formula

C31H44N6O8

Molecular Weight

628.72

Purity by HPLC

>95 % (214 nm)

Appearance

Pale yellow solid

Solubility

100 µM in 2% (v/v) DMSO or 2% (v/v) Ethanol / aqueous buffers. Pre-dissolve the complete 10 mg (adjust to the weight given on the vial) in 318 µl DMSO or EtOH (50 mM). DMSO stock solutions are sterile and can be stored at -20°C for at least 6 months. To avoid too many freeze-thaw cycles, we strongly recommend storage of aliquots. Take e.g. 10 µl of the 50 mM stock solution and add 90 µl DMSO (or EtOH) to obtain a 5 mM stock solution - dilute by adding 4.9 ml buffer or assay components to obtain your final assay solution (100 μM). In case your assay or experimental setting does not tolerate these DMSO levels, we recommend the following procedure: Pre-dissolve the complete 10 mg (adjust to the weight given on the vial) in 159 µl DMSO (100 mM). Make sure that the compound is dissolved properly. Take e.g. 10 µl of the 100 mM stock solution and add 9.99 ml buffer/ assay components (1:1,000) to obtain your final assay solution (100 μM). In our experience, the compound dissolves in Tris-buffered saline even if locally a milky precipitate may form initially.

Application

Site specific inhibitor of tissue transglutaminase IC50 ~ 0.02 µM[1] Cell permeable at 40 µM[1]

Storage

Store at -20°C, desiccate

Reference(s)

[1] Schaertl, S. et al. J. Biomol. Screen. 2010, 15, 478. [2] Verhaar, R. et al. Neurochem. Int. 2011, 58, 785. [3] McConoughey, S.J. et al. EMBO Mol. Med. 2010, 2, 349. [4] Lauzier, A. et al. Arthritis Res. Ther. 2012, 14, R159. [5] Verhaar, R. et al. Neurochem Int. 2013, 62, 486. [6] Fischer J. et al. J. Invest. Dermatol. 2013, 133, 1170. [7] Lauzier, A. et al. Arthritis Research & Therapy 2012, 14, R159. [8] Wang Z. et al. Cell Death & Dis. 2013, 4, e808. [9] de Jager, M. et al. J. Neurochem. 2015, 134, 1116. [10] de Jager, M. et al. Neuropathol. Appl. Neurobiol. 2016, 42, 255. [11] Wilhelmus, M. et al. Sci Rep. 2016, 6, 20569. [12] van der Wildt, B. et al. Nucl. Med. Biol. 2016, 43, 232. [13] Recktenwald, C. et al. J. Biol. Chem. 2016, 291, 13580. [14] Singh, G. et al. J. Biol. Chem. 2016, 291, 9119. [15] Espitia Pinzon, N. et al. Sci Rep. 2017, 7, 40995. [17] Katt, P.W. et al. Mol. Pharmaceutics 2015, 12, 46-55. [18] Algarni A.S. et al. Biochem. Pharmacol. 2017, 128, 55-73. [19] Chrobok, N.L. et al. PLoS ONE 2018, 13, e0209522. [20] Selcuk, K. et al. Matrix Biology 2024, 125, 113-132.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T002: Human tissue transglutaminase
Z011: Z-(D)-DON-Val-Pro-Leu-OMe, Z-(D)-DON
B003: Boc-DON-Gln-Ile-Val-OMe, Boc-DON