Protein labeling using transglutaminase (TGase) is a smart enzymatic labeling alternative to chemical protein labeling procedures.
or site-directed protein labeling with fluorescent dyes
are common applications of TGase labeling.
Advantages of transglutaminase (TGase) mediated site-specific protein labeling: Zedira’s protein labeling tools
are available as
- TGase Protein Labeling Kits
- Individual components
- TGase Labeling Service
Principle of TGase-catalyzed protein labeling, protein modification and protein conjugation
- Defined degree of labeling
- Defined label position(s)
- Homogenously labeled protein
- Higher solubility in water
- Minimized amounts of unlabeled protein
- No or reduced impact on bioactivites of labeled proteins
Requirements for transglutaminase labeling
TGases catalyse the acyl transfer reaction between the γ-carboxyamide group of protein-bound glutamine residues and the ε-amino group of protein-bound lysine or primary amines. Thus, primary amines as well as glutamine-containing peptides may carry a broad variety of labels like biotin, PEG or fluorescent dyes etc.
Transglutaminase protein labeling requires substrate sequences on the target protein surface, which are in general not abundant on proteins:
- Accessible lysine residues
- or accessible glutamine residues
- otherwise transglutaminase substrate sequence tags can be introduced recombinantly, if no lysine or glutamin residues are accessible
Examples for utilization of modified proteins
- Fluorescence-based HTS-assay
- Diagnostics (antibodies, antibody-bindingproteins, lectins etc.)
- Therapeutic proteins (PEGylation)
- Protein immobilization (after biotinylation)
Please find information about TGase Protein Labeling Kits
, the individual components and Zedira’s TGase Labeling Service
in the respective categories.
In case of any questions just contact us!