Glutamine-donor peptides

Art. No.

C092

Synonym

Z-Gln-Gly-(PEG1,088), monodisperse

Molecular Formula

C64H118N4O29

Molecular Weight

1407.63

Purity by HPLC

>90 % (214 nm)

Appearance

Off-white solid

Reconstitution

e.g. for the example application given below, 5.63 mg Z-Gln-Gly-(PEG1,088) were dissolved in 1 mL buffer (20 mM NaOAc, pH6) to obtain a 4 mM stock solution

Application

PEGylated glutamine donor substrate for transglutaminases / Protein labeling A 29 kDa-protein (component CK from Substrate Finder Kit L001) is labeled with Z-Gln-Gly (PEG1,088) using MTG (microbial transglutaminase) according to a modified protocol of the Please see also MTG catalyzed PEGylation using (PEG1,088)amine (P010), (PEG5,000)amine (P011), or Z-Gln-Gly-(PEG5,000) (C093) as substrate in the corresponding product data sheets. Substrate Finder Kit (L001). The result shown in Figure 1 reveals the increase in molecular weight of the protein (black arrow) up to the covalent incorporation of Z-Gln-Gly-(PEG1,088) (red arrow).

Storage

Store at -20°C, desiccate

Reference(s)

Case, A. et al. Biochemistry 2007, 46, 1106. Mero, A. et al. J. Controlled Release 2011, 154, 27.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T300: Andracon® – Recombinant Microbial Transglutaminase
P010: (PEG1,088)amine, monodisperse
P011: (PEG5,000)amine, polydisperse
C093: Z-Gln-Gly-(PEG5,000), polydisperse
L001: Substrate Finder Kit