Back to all products
| TGase Protein Labeling Kits |
| Transglutaminase |
| Reaction stopper |
| Amine-donors |
| Glutamine-donor peptides |
| TGase Labeling Service |
Protein Labeling
Protein labeling using transglutaminase (TGase) is a smart enzymatic labeling alternative to chemical protein labeling procedures.
Protein biotinylation, PEGylation or site-directed protein labeling with fluorescent dyes are common applications of TGase labeling.
Advantages of transglutaminase (TGase) mediated site-specific protein labeling:
Principle of TGase-catalyzed protein labeling, protein modification and protein conjugation
Requirements for transglutaminase labeling
Transglutaminase protein labeling requires substrate sequences on the target protein surface, which are in general not abundant on proteins:
Examples for utilization of modified proteins
Please find information about TGase Protein Labeling Kits, the individual components and Zedira’s TGase Labeling Service in the respective categories.
In case of any questions just contact us!
Protein biotinylation, PEGylation or site-directed protein labeling with fluorescent dyes are common applications of TGase labeling.
Zedira’s protein labeling tools are available as
- TGase Protein Labeling Kits
- Individual components
- TGase Labeling Service
Advantages of transglutaminase (TGase) mediated site-specific protein labeling:
- Defined degree of labeling
- Defined label position(s)
- Homogenously labeled protein
- Higher solubility in water
- Minimized amounts of unlabeled protein
- No or reduced impact on bioactivites of labeled proteins
Principle of TGase-catalyzed protein labeling, protein modification and protein conjugation
TGases catalyse the acyl transfer reaction between the γ-carboxyamide group of protein-bound glutamine residues and the ε-amino group of protein-bound lysine or primary amines. Thus, primary amines as well as glutamine-containing peptides may carry a broad variety of labels like biotin, PEG or fluorescent dyes etc.
Requirements for transglutaminase labeling
Transglutaminase protein labeling requires substrate sequences on the target protein surface, which are in general not abundant on proteins:
- Accessible lysine residues
- or accessible glutamine residues
- otherwise transglutaminase substrate sequence tags can be introduced recombinantly, if no lysine or glutamin residues are accessible
Examples for utilization of modified proteins
- Fluorescence-based HTS-assay
- Diagnostics (antibodies, antibody-bindingproteins, lectins etc.)
- Therapeutic proteins (PEGylation)
- Protein immobilization (after biotinylation)
Please find information about TGase Protein Labeling Kits, the individual components and Zedira’s TGase Labeling Service in the respective categories.
In case of any questions just contact us!
| Phone: | +49 6151 66628-0 | |
| E-mail: | contact@zedira.com |
Discover Our New Catalogue Edition and Dive into the World of Transglutaminases! 
Successful ISO9001:2015 recertification 
Dr. Falk Pharma and Zedira announce successful completion of the phase 2a proof-of-concept study of ZED1227 for the treatment of Celiac Disease 
Dr. Falk Pharma und Zedira verkünden den erfolgreichen Abschluss der Phase 2a-Studie mit ZED1227 zur Behandlung von Zöliakie 
Reversibly acting transglutaminase 2 inhibitors: drug candidates for the treatment of fibrosis 
Transglutaminase antibodies and neurological manifestations of gluten sensitivity 
Design of Oral FXIIIa Blockers as Safer Anticoagulants Mission Impossible? 
Microbial transglutaminase (MTG) enables efficient and site-specific conjugation to native antibodies without the need of antibody engineering 
Tridegin as FXIIIa inhibitor 
Microbial transglutaminase: from discovery to market 
Tissue transglutaminase inhibitors 
Tissue transglutaminase in Alzheimers Disease 
Factor XIIIa: novel target for anticoagulation? 
Microbial transglutaminase for site-specific protein conjugation