Transglutaminase 2

Art. No.

T106

Synonym

Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase

Molecular Weight

~80 kDa (varies due to Degree of Labeling (see CoA))

Source

Recombinant produced in E. coli

Purity

> 95 % (visually by SDS-PAGE)

Activation

Add 10 mM Ca2+ to activate His6-rhTG2.

Activity

> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)]. 1 U is defined as the increase in fluorescence intensity of 1 a.u./min.

Appearance

Liquid.

Application

His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.

Storage

Store at -20 °C in working aliquots. Repeated freezing and thawing is not recommended.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T002: Human tissue transglutaminase
A033: Monoclonal antibody to human TG2 (Catalytic Domain, clone XTG17)