Recombinant Gliadin Peptides

Art. No.

G055

Synonym

Carrier protein control

Background info

Gliadin peptides derived from Triticum aestivum (wheat) are the main immunotoxic antigens present in celiac disease. They are substrates for tissue transglutaminase, which specifically deamidates glutamine residues within these peptides, and therefore strongly increases their immunogenicity (Dørum S. et al., J. Proteome Res. 2009; 8:1748-55). We offer a set of fusion proteins that carry the different native and deamidated peptide sequences that facilitate the analysis of antibodies found in celiac disease related samples.

Molecular Weight

22 kDa

Source

Recombinantly produced in E. coli

Purity

> 95% (SDS-PAGE and Coomassie staining)

Appearance

White lyophilized solid.

Description

Carrier protein control consists of the carrier protein, which is used for the expression of G005, G006, G007, G051, G052, and G054. It can therefore be used to rule out unspecific binding of antibodies to the carrier protein itself.

Reconstitution

Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. Further dilutions can be made in your buffer of choice. After reconstitution, the solution should be stored frozen in working aliquots.

Application

The recombinant antigen is meant for solid (ELISA and immunoblot) and fluid phase assays as well as Western Blotting.

Coating

Dilute with your coating buffer to an appropriate concentration e.g. 1 µg/ml. Please notice that coating conditions have to be evaluated carefully.

Storage

Store at -80°C. Stability is given for at least 5 years when stored at -80°C (see retest date on Certificate of Analysis), with potential to date extend after retesting. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Solutions are stable for at least 2 years when stored at ≤ -20°C. Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Avoid repeated freezing and thawing. Delivery is possible at ambient temperature

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

G005: DGPx4
G006: DGPx2
G007: DGPx1
G051: 26mer gliadin peptide
G052: 33mer gliadin peptide
G054: 33mer DGP
G056: DGP and Gliadin peptides (Set No 1)