Recombinant Gliadin Peptides

DGP and Gliadin peptides (Set No 1)

(G051, G052, G007, G054 and G055, 100 µg each)

1 Set 740 € available

Art. No. G056
Source Recombinantly produced in E. coli
Purity > 95% (SDS-PAGE and Coomassie staining)
Appearance White lyophilized solid
Description The set comprises the following products:
G051 26mer gliadin peptide (native 26mer gamma gliadin peptide)
G052 33mer gliadin peptide (native 33mer alpha gliadin peptide)
G007 DGPx1 (deamidated 26mer gamma gliadin peptide)
G054 33mer DGP (deamidated 33mer alpha gliadin peptide)
G055 Carrier protein control
Reagents The recombinant proteins are lyophilized from a solution of ~50 mM NaH2PO4, pH 6.8
Reconstitution Add the volume of H2O the proteins are lyophilized from (see Certificate of Analysis) to each vial of lyophilized powder. Rotate vials gently until solid dissolves. Further dilutions can be made in your buffer of choice. After reconstitution, the solutions should be stored frozen in working aliquots.
Application The recombinant antigens are meant for solid (ELISA and immunoblot) and fluid phase assays as well as Western Blotting.
Coating Dilute with your coating buffer to an appropriate concentration, e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature.


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