Transglutaminase 2

Art. No.

T167

Synonym

Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase

Molecular Weight

53.3 kDa

Source

Recombinant produced in E. coli

Activation

The Transglutaminase is activated with 10 mM Ca_²⁺.
Important note: In order for the protein to be catalytically active, it is necessary to incubate the working solution with a reducing agent, preferably 20-30 mM DTT, for at least 30 min at room temperature, immediately before use.

Activity

Activity is determined by measuring the rate of fluorescence enhancement after rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λ_ex = 332 nm, λ_em = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Appearance

White lyophilized solid

Description

Short rhTG2 is a truncated variant of TG2 lacking both ß-barrel domains. The protein was expressed in E. coli based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). It is N-terminally fused to a hexahistidine-tag. Short rhTG2 was purified by ion metal chelating chromatography (IMAC).

Application

rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. rhTG2 may also be used for immunoprecipitation.

Storage

Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.

Reference(s)

Stamnaes et al, PLOS ONE, 2015, 10:e0134922

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T002: Human tissue transglutaminase